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| Soares,Taliane Leila; Jesus,Onildo Nunes de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de. |
| The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passion fruit; Pollen germination; Histochemical analysis; Wild passion fruit. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000400023 |
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| Soares,Taliane Leila; Souza,Everton Hilo de; Costa,Maria Angélica Pereira de Carvalho; Silva,Sebastião de Oliveira e; Santos-Serejo,Janay Almeida dos. |
| ABSTRACT Obtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB) by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride). The pollen tube growth was evaluated by germinating grains in three culture media — M1: 0.03% Ca(NO3)∙4H2O, 0.02% Mg(SO4)∙7H2O, 0.01% KNO3, 0.01% H3BO3 and 15% sucrose; M2: 0.03% Ca(NO3)∙4H2O, 0.01% KNO3, 0.01% H3BO3 and 10% sucrose; and M3: 0.015% H3BO3, 0.045% Ca3(PO4)2 and 25% sucrose. The acetocarmine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa L.; In vitro pollen germination; Histochemical analysis; Plant breeding. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000200145 |
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